Helmet is required to be able to go underwater, but once there the helmet can be removed. However, without it players will take 5 times 20 Drowning damage kicking in after four seconds of standing still. It is useful out of the depths, too: some monsters, like Water Elementals use drowning damage attacks.
That means you won't be able to go underwater if you not wearing it :)
Source : TibiaWiki
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